Cell is capped at day nine or ten, correct.. There is some controversy about the effectiveness of OAV on mites in cells with Larvae of five days or older.. I know the vapor gets into those cells, it gets into EVERYTHING as easily as smoke... Do the young mites move OUT from under the larvae? Are the crystals still present when the mites are old enough to move about more?
We still have a lot to learn... The very basics of it is, that OAV does not affect the bees, the honey, the wax etc, EVEN when tested by treating once a week for an entire winter.. the bees came out of winter strong and healthy and the hive was booming in no time.
In effect, you could treat at day 0 which could be considered day nine, and then treat again on day 12, which would be day 21 to the larvae that were capped the first time you treated, and catch the mites emerging from those cells.
I know beeks that treat ONLY ONCE in the spring, and only once in the fall, and i am keeping close tabs on two of them, just to monitor their mite levels and see how their treatment schedule works..
I only treat once in the spring, usually as early as possible, so there is less brood... Possible to me = when i dont have to worry about shivering any more....
BUT, in the fall, I want to make SURE the bees that will be my OVERWINTERED BEES are well treated and as healthy as possible...
Take care of the bees, that take care of the bees that will be going into winter. Larry Connor catch phrase.
In effect, you treat the bees that you want to be as healthy as possible, so that they can make sure the next generation of bees is as healthy or healthier than they were, so that those bees will then insure the bees that will live all winter in your hives are as healthy, mite, and disease free as they can be... his philosophy is, that a sick bee will not care for another new bee as well as a good healthy bee can or will.
So I treat three times, to KILL as many mites as I can. Day 0, Day 7, SKIP, and day 21. I may not NEED to do that, but I can say it works VERY VERY well, so until I have been shown a better way I will stick to doing that.