Yes they are a 1 treatment, It has been approved for use when the honey supers are on, and has been proven effective with the extra space. For normal evaporation of formic to work the bees need to be confined down to the cluster space of the bees for the treatment to be effective so treatments were often delayed till after the honey supers were removed for extraction. The gel that holds the formic to allow it to slowly be released also allow the formic to enter the brood cells thru the cappings killing the mites that are in the cells. after a week whats left of the gel packs can be disposed of or they can be left in the hive and the bees will eventually remove them.