yeah blue, your welcome. i love these kinds of pic challenges. i looked at that late last night, downloaded your picture and enlarged it. when i first looked at it, my eyes were first drawn to that bee. when i looked at her up close, it was the thorax and the leg structure. not sure how to explain this, robo said it best i guess, the hair on the thorax....queens have more of a shiny thorax to them, like robo said, no hair, and also like i said the legs.
it is hard picking them out, but you know what, you're doing great, and looking forward to your updates on this colony and what you find.....