Yes. When i go to see the kids a tthe libraries or schools, that is what I do.. Slip the frames in and close it up. The reaction you get when you slide the board out and they see all those bees is wonderful. Sometimes I make sure there is a marked queen that is easy to find, if I know I will not be gone more than a couple hours.. If it will be an all day thing I dont take the queen, OR, when i return I put a nuc on the top and newspaper combine them.. it may not be necessary, but if the queen is marked, its because she is VSH, Hygienic, survivor etc, and I dont want to lose her.