You can bee successful with dribbling down to -3ºC = 26.600ºF without any problems.
I wouldn't use the green plastic frame, better to use an empty frame and let them build freely, this is better beecause more Young worker bees are involved and the mites are more likely to bee on them.
The third frame in a ten frame box early in the spring build up as a drone frame catches mites successfully.
Cut out and freeze the capped drone brood, save and feed to birds in Winter or give to chickens that go nuts with delight and love eating larvae.

mvh Edward
