if they turn their butts in the cage trying to sting her, or what looks like biting, or are acting aggressively or agitated not a good sign.
also, this can be coupled with the 'sounds' or a 'hum' we learn to recognize or associate with what we think bees are.... happy or agitated, and activity on the combs.
if you see bees clamoring on the cage with their antennae in, AND you witness bees fanning near and from a distance of the cage with their butts in the air, this is typically a good sign........other bees will quickly come to the tops of the frames, and your frames will be lined with bees at the tops.
kinda cool to see actually. a new queen reigns.
i think setting the cage down with the queen on top of the frames and watching how the bees react is a good indication on how well they will accept her.