Just saw this and thought I'd offer my 2 cents (wind is bowing the wrong direction for me to be in my deer blind!). There are many different ways (and opinions
) to produce cells for queen production. I have a very good friend that produces 60-80,000 cells each spring and he has a very simple method that works well. He selects his breeder colonies from double deep hives. He places a double screen between the two boxes and makes sure the queen is in the bottom box. The double screen only has to be thick enough to keep bees from orally passing queen substance from the bottom box to the top box. Consequently, the bees in the top box think they have no queen and they are highly motivated to produce a replacement queen; he uses that box to for producing cells from start to finish. The bottom box is used to harvest young larvae for grafting. Moving comb up with older and capped brood keeps the nurse bee numbers high in the top box and moving empty (of young brood) combs from the top to the bottom box provides new places for the queen to lay and keep the supply of young larvae for grafting high to sustain cell production. In practice, combs used to graft queens are returned to the top box after all "graftable" larvae have been removed and the empty combs they displace are moved to the bottom box for the queen to lay more eggs. The top box has to be checked for cells produced from recycled comb but that's fairly easy since they are placed in the center of the top box in a "known" position so it isn't necessary to check all the frames of comb for cells. Since I've retired from keeping too many bees
, this is a method I plan to use. Another beauty of the technique is that the 2 boxes can be reunited at anytime; no need to go the newspaper route etc. Both boxes smell the same because they are the same hive, just separated vertically. Raise the queens you need, take the screen out and it's business as usual.