From the research I have seen, it is recommended to treat when the bees ARE in cluster because there is little to no, or very little capped brood...
From Randy Olivers Site;
Does vaporization hurt the bees?
Radetzki didn’t note increased bee mortality after winter treatment. Heinz Kaemmerer of Heilyser Technology Ltd. says: “We treated several colonies for 3 months during winter, once a week with the vaporizer and all colonies survived.” “With brood, colonies can be treated with the right amount of OA 3 to 4 times, a week apart; there is no harm to bees, queen or brood.” Medhat Nasr confirms that vaporized oxalic is very gentle to the bees.
//end
The transfer rate of the crystals is very high. Dividing a hive in half, treating one side, and then removing the divider showed a 70% transfer rate of the OA crystals and an equal decimation of the mites. So treating while in cluster, I imagine that the crystals will be transferred into the cluster as the bees move in and out... Is it as effective as when treating during warmer weather? I woulod think so, because there is no capped brood.. but thats just speculation on my part.
I dont treat when clustered, I do it as Lee says.. Mostly because it still works very well, and I dont have to freeze important parts of my anatomy off to do it, OR disturbn the bees while they are clustered.
As Iddee said, the temp inside the hive is probably not hotter than outside. if its 100 degrees with the sun shining on the hive they are evaporating water to cool the hive, and they are keeping the hive warm if its cooler than 96 (ish) degrees.. OAV works byu sublimation. The heat turns the crystal into a liquid for JUST a moment, and then it is vaporized.. that vapor turns back into crystals as it cools so no matter if the temp is 20 degrees or 100 degrees, it should not effect how the OA crystallizes to any extent that would make it either NOT work, or work too well. The only consideration might be, how much capped brood do you have? If its a bunch, treating again after the capped brood emerges (12 days, give or take a day) should catch the mites that were hiding under the cappings when you treated the first time.
another study;
Their findings were that beekeepers need only carry this treatment out once a year, because the treatment reduces the number of mites so dramatically that it takes them a long time to build back up.
Web sites can be found on my site here;
http://www.outyard.net/treatments.html So we have research groups, treating for 12 weeks in a row during winter, and research groups treating ONCE in summer, and both found astonishing results of 97% or more mite kills... With 0 bee mortality....
So far so good, its "still" looking like the proverbial silver bullet.