Vincent, the replies above all have the wisdom of experience (frequently obtained from mistakes).
I found
THIS video yesterday. It shows good basic manipulation techniques for a hive inspection (he isn't using an inner cover though, not many bees either). Dr. Wilson of the USDA Honey Bee Lab in Laramie, Wyoming (1976) taught me the same basic procedure and hive tool use, its the procedure to use.
Wearing at least a veil or bee jacket, use this procedure with Jen's tool for removing the first frame and, moving slowly, you should get through the inspection. On those days you should leave the bees alone, they will tell you right away.....be listening and watching. Think fast, move slow!
BTW, the queen can be on any frame in the hive, even out sunning herself on the front landing. Treat every frame like the queen could be on it, even look at the bottom of the inner cover until you see her, only then you know where she is and can work accordingly. HTH :-)